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Part:BBa_K415039

Designed by: Shawn Pan   Group: iGEM10_MIT   (2010-10-26)


pBR322 Derived Low Copy pMB1 Origin Operon

This origin is derived from the low (~20) copy pBR322 plasmid. The pMB1 replication origin for pBR322 is a regulated version of the high copy pUC19 origin (see I50022). The copy number is regulated by a small protein ROP. This part contains the origin with the appropriate copy-number regulatory domains, and can be used with the standard biobrick base vector I51020 to construct biobrick backbones. The part contains a RBS before the ROP protein but not a promotor.

Note: This part contains an VR sequencing site between the ROP and origin, due to its homology with the pUC19 origin. This site should ideally be removed in a revised version.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 454


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